Amperometric Biosensors for L-Arginine Determination Based on L-Arginine Oxidase and Peroxidase-Like Nanozymes

There are limited data on amperometric biosensors (ABSs) for L-arginine (Arg) determination based oxidases that produce hydrogen peroxide (H2O2) as a byproduct of enzymatic reaction, and artificial peroxidases (POs) decomposition H2O2. The most frequently proposed Arg-sensitive oxidase-based ABSs contain at least two enzymes in the bioselective layer; this complicates procedure increases cost analysis. Therefore, construction one-enzyme ABS Arg analysis is practical problem. In current work, fabrication, characterization three types direct measurement were proposed. oxidase (ArgO) isolated from mushroom Amanita phalloides was co-immobilized with PO-like nanozymes (NZs) surface graphite electrodes. As PO mimetics, chemically synthesized NZs CeCu (nCeCU) NiPtPd (nNiPtPd), well green-synthesized hexacyanoferrate copper (gCuHCF), used. novel exhibited high sensitivity selectivity to Arg, broad linear ranges good storage stabilities. Two tested real samples products containing including pharmaceutical preparation “Tivortine”, juices, wine. A correlation (R = 0.995) demonstrated between results testing “Tivortine” juice using nCeCU/GE nNiPtPd/GE. It worth mentioning only slight difference (less than 1%) observed “Tivortin” experimentally determined content its value declared by producer. ArgO-NZ-based may be promising different branches science, medicine, industry.